G-quadruplex formation using fluorescent oligonucleotides as a detection method for discriminating AGG trinucleotide repeats

We have developed a simple and sensitive system for detecting AGG trinucleotide repeats through the formation of intermolecular G-quadruplexes using a fluorescent oligonucleotide. The fluorescence signal increased rapidly and dramatically by 44.7-fold with respect to the low background signal in the presence of RNA agg repeats and by 35.0-fold in the presence of DNA AGG repeats.1163Ysciescopu

An Automated WSDL Generation and Enhanced SOAP Message Processing System for Mobile Web Services

Web services are key applications in business-to-business, business-to-customer, and enterprise applications integration solutions. As the mobile Internet becomes one of the main methods for information delivery, mobile Web Services are regarded as a critical aspect of e-business architecture. In this paper, we proposed a mobile Web Services middleware that converts conventional Internet services into mobile Web services. We implemented a WSDL (Web Service Description Language) builder that converts HTML/XML into WSDL and a SAOP (Simple Object Access Protocol) message processor. The former minimizes the overhead cost of rebuilding mobile Web Services and enables seamless services between wired and wireless Internet services. The latter enhances SOAP processing performance by eliminating the Servlet container (Tomcat), a required component of typical Web services implementation. Our system can completely support standard Web Services protocol, minimizing communication overhead, message processing time, and server overload. Finally we compare our empirical results with those of typical Web Service

Engineering de novo disulfide bond in bacterial alpha-type carbonic anhydrase for thermostable carbon sequestration

Exploiting carbonic anhydrase (CA), an enzyme that rapidly catalyzes carbon dioxide hydration, is an attractive biomimetic route for carbon sequestration due to its environmental compatibility and potential economic viability. However, the industrial applications of CA are strongly hampered by the unstable nature of enzymes. In this work, we introduced in silico designed, de novo disulfide bond in a bacterial alpha-type CA to enhance thermostability. Three variants were selected and expressed in Escherichia coli with an additional disulfide bridge. One of the variants showed great enhancement in terms of both kinetic and thermodynamic stabilities. This improvement could be attributed to the loss of conformational entropy of the unfolded state, showing increased rigidity. The variant showed an upward-shifted optimal temperature and appeared to be thermoactivated, which compensated for the lowered activity at 25 degrees C. Collectively, the variant constructed by the rapid and effective de novo disulfide engineering can be used as an efficient biocatalyst for carbon sequestration under high temperature conditions.1194Ysciescopu

An enhancement of optimized detection rule of security monitoring and control for detection of cyberthreat in location-based mobile system

A lot of mobile applications which provided location information by using a location-based service are being developed recently.For instance, a smart phone would find my location and destination by running a program using a GPS chip in a device. However,the information leakage and the crime thatmisused the leaked information caused by the cyberattack ofmobile information systemoccurred. So the interest and importance of information security are increasing. Also the number of users who has used mobiledevices in Korea is increasing, and the security of mobile devices is becoming more important. Snort detection system has beenused to detect and handle cyberattacks but the policy of Snort detection system is applied differently for each of the different kindsof equipment. It is expected that the security of mobile information system would be improved and information leakage would beblocked by selecting options through optimization of Snort detection policy to protect users who are using location-based servicein mobile information system environment in this paper

Adjunctive herbal medicine therapy for inflammatory bowel disease: A systematic review and meta-analysis

© 2017 Elsevier GmbH Introduction We conducted a systematic review and meta-analysis to evaluate the evidence for herbal medicine for inducing or maintaining remission in patients with ulcerative colitis (UC) and Crohn's disease (CD). Methods Eight databases were searched up to January 2017 for randomised controlled trials of herbal medicine as an adjunct to conventional medication in patients with inflammatory bowel disease (IBD). Data were extracted to obtain risk ratio (RR) of failure of inducing or maintaining remission, with 95% confidence intervals (CI). Risk of bias was assessed using the Cochrane criteria. Results Twenty-nine RCTs (24 UC, 5 CD) were included. In UC, herbal medicine was superior to placebo for clinical remission (RR of remission failure = 0.74, 95% CI: 0.59–0.93; I2 = 62%) and maintaining remission (RR of failure to maintain remission = 0.34, 95% CI: 0.17–0.67; I2 = 0%). Traditional Chinese patent medicine with standard therapy reduced the risk of no comprehensive remission by 19% compared to standard therapy alone (RR of no remission = 0.81, 95% CI: 0.70–0.95; I2 = 0%). In CD, however, the effect of herbal medicine was significant neither for inducing nor maintaining remission (RR of remission failure = 0.57, 95% CI: 0.24–1.33; I2 = 87%; RR of failure to maintain remission = 0.95, 95% CI: 0.60–1.52). Few serious adverse events were reported. Conclusions An adjunctive herbal medicine compared to standard therapy appears effective with few adverse events in achieving and maintaining remission in UC, while there is a lack of supporting evidence for CD. Future high quality trials are warranted

Sorghum cobalt analysis on not determined wave length with atomic absorption spectrophotometer on background correction mode

This study was to know the better wave length on measuring cobalt content in forage sorghum hybrid (Sorghum bicolor) with an atomic absorption spectrophotometer. The analysis was on background correction mode with three wave lengths; 240.8, 240.7 (determined wave length or recommended wave length) and 240.6 nm, respectively. The larger absorbance value on the 240.7 nm, apparently, it might be considered as a good wave length but the smaller background value was a more important factor for the analysis as was shown on 240.6 nm. Correlation coefficients between the values on 240.7 nm: 240.6 nm and between them (240.8 nm: 240.6 nm) were higher and this common 240.6 nm was considered the better wave length.Key words: Atomic absorption spectrophotometer; background correction mode, cobalt analysis, forage sorghum, not determined wave lengths

Effect of amorphous Si quantum-dot size on 1.54 μm luminescence of Er

The role of the size of amorphous silicon quantum dots in the Er luminescence at 1.54 μm was investigated. As the dot size was increased, more Er ions were located near one dot due to its large surface area and more Er ions interacted with other ones. This Er-Er interaction caused a weak photoluminescence intensity, despite the increase in the effective excitation cross section. The critical dot size needed to take advantage of the positive effect on Er luminescence is considered to be about 2.0 nm, below which a small dot is very effective in the efficient luminescence of Er. © 2005 The Electrochemical Society. All rights reserved